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The weight of the patient should also be incorporated in the calculations because it has a direct impact on the dose and the volume of distribution. The volume of distribution is calculated using the formula:

The surface area of the area under the curve (AUC) is another parameter that is routinely measured in PK/PD studies. Again, it is easier to use cm instead of mg. The AUC can be calculated using a well-known formula, but there are several other methods available [69].

Another important parameter to consider when calculating the T > MIC is the maximum plasma concentration (Cmax). When calculating T > MIC, the AUC is divided by the Cmax (AUC/Cmax) or the AUC is multiplied by the Cmax (AUC x Cmax). The problem with the first option is that it ignores the concentration of the drug at the MIC. The second solution is the most common one for the calculation of T > MIC and is the one we recommend because it considers the drug concentration at the MIC (Table 4).

The AUC and Vd typically have a large variability. As a result, there are several methods available to quantify the data and to identify the optimal target that will lead to the best result, for example, the MED or the MRTD. All available methods that report the AUC are offered in the Excel spreadsheet, but the tRPS is also available in a different way [Table 4], which is specific to the tRPS (http://www.datapharm.com/tRPS/). It is also possible to select the median or the mean and then calculate the T > MIC for each of the selected dose ranges (Table 4).

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