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The first five classes of glycan structures (listed in Table 1) were chosen because they represent the five most commonly found structures in recombinant protein biologics [41]. The GlycoSyn database [42] was consulted to determine the most common glycoforms in biologics ( http://www.glycosyn.com/glycosyn-database.html ), and the seven glycan structures representative of those most commonly found in this database were selected for the analysis (listed in Table 2). The glycan structures were specified by the glycan core structure and the quantifiable number and distribution of attached sugar moieties (“fucosylation,” “galactosylation,” “sialylation,” “N-acetylation,” “O-acetylation,” “fucosylated O-acetylation,” and “galactosylated O-acetylation”).
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